Pigeon DNA identification instrument

Introduction:

For a long time, having a good lineage has been considered an important factor affecting competition results. The so-called good lineage refers to a specific genotype that can be inherited. Given that certain genes in racing pigeons have a significant impact on their athletic ability, through reference to authoritative literature and European testing experience, combined with existing innovative technologies, it has been found that the homing ability (DRD4) index, endurance (LDHA) index, and cryptochrome (CRY1) index play important reference roles in breeding pigeons for their flying ability, By detecting the above indicators, a more scientific reference can be provided for pigeon enthusiasts in choosing racing and breeding pigeons.

Our pigeon flight ability identification device, developed based on the aforementioned genetic research, can simultaneously identify genes that affect flight ability, including gender identification, homing ability DRD4, endurance LDHA, navigation ability, and circadian rhythm CRY1.

Basic structure of the instrument：

The real-time fluorescence quantitative PCR system is an instrument for real-time detection of reactions, mainly composed of gene amplification thermal cycle system, fluorescence real-time detection system, microcircuit control system, computer and application software. Two core functional modules are the thermal cycling system and the fluorescence real-time detection system. The working principle of the gene amplification thermal cycle system is basically the same as that of traditional gene amplification instruments, using semiconductor heating and cooling to complete the thermal cycle process. The fluorescence detection system mainly consists of fluorescence excitation components, light signal transmission components, fluorescence detection components, and control systems.

Instrument features:

1. Small size, light weight, easy to carry, compatible with 0.2 PCR single tube and eight tube;

2. Adopting the American MARLOW customized Peltier module high-level semiconductor chip and the new generation of semiconductor temperature rise and fall technology, the fastest temperature change rate can reach 7 degrees per second, and the service life can reach one million cycles;

3. Adopting unique lighting detection and processing technology, automatically adjusting the fluorescence background, improving fluorescence signal sensitivity and signal-to-noise ratio, and achieving better results;

4. 13S four channel fast lighting, stable fluorescence acquisition signal, and reduced delay error;

5. Adopting maintenance free and long-life LED light sources that do not require replacement, with independent fluorescent channels and less crosstalk between different channels;

6. Constant current control circuit, smooth power output, extended Peltier lifespan, and improved temperature control accuracy;

7. Has protection functions such as overcurrent and over temperature;

8. Powerful software analysis functions that can perform quantitative analysis, melting curve analysis, gene typing, etc. The analysis software is upgraded for free for life and supports software customization for different industries;

9. Equipped with a 10 inch color touch screen control.

Technical parameters:

External dimensions: 235mm * 385mm * 175mm (width * depth * height)

Weight: 5.8Kg

Electrical parameters:~220V/50Hz, 255W

Data interface: USB 2.0 * 2

Operating conditions: Temperature: 10-30 ℃, Humidity: 20%~80%

Transportation and storage conditions: temperature: -20~55 ℃, humidity: 20%~80%

Altitude:<2500 meters

Noise level: A-weighted,<60dB

Sample size: 32 * 0.2mL

Test tube type: 0.2PCR single tube, eight row tube

Sample volume: 15-100uL

Heating/cooling method: semiconductor heating/cooling

Temperature range: 4 ℃ -99 ℃

Maximum heating rate: ≤ 5.5 ℃/s (MAX)

Average heating rate: ≥ 3.5 ℃/s

Maximum cooling rate: ≤ 4.5 ℃/s (MAX)

Average cooling rate: ≥ 2.5 ℃/s

Temperature control accuracy: ≤ ± 0.01 ℃

Temperature accuracy: ≤ ± 0.1 ℃

Temperature uniformity: ≤ ± 0.3 ℃

Fluorescence detection channels: 4 channels

Light emitting device: High brightness LED

Daylighting device: high sensitivity, high signal-to-noise ratio photodiode

Adapted probe or dye: First channel: 470/520 FAM, SYBR Green

Second channel: 530/570 HEX, JOE, VIC

Third channel: 580/610 ROX, CY3.5, Texas Red

Fourth channel: 630/670 CY5

Detection sensitivity: 1 copy

Linear detection range: 100-1010 copies

Linear correlation coefficient: ≥ 0.999

Channel crosstalk: No crosstalk

Detection repeatability: ≤ 1.0%